Olympus IX81 TIRF Microscope

This microscope (5208) can image samples using brightfield, DIC, phase contrast, epifluorescence and Total Internal Reflection microscopy. Below you can find information on our microscope and tutorials for image anlaysis.

Use the following table for converting your image into real distances

Metamorph Users Guide

Calibration Table.docx
Olympus IX81 Training Document.pdf

If you are looking to count nanosheets, here is a tutorial using imagej.

I also recommend FIJI, which is the same as Imagej, but with many plugins pre-installed.

Counting Sheets